| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Bacillus smithii | CCCATTCCTCATTTGCCTGC | AACGGGTTCCCTCGTAAGC | 59.54 | 59.71 | 247 | 168 |
100.00%
|
100.00%
|
| Primer 2 | Bacillus smithii | AACGAATCCCCTCCAATGGA | CGCATTTTGTTCAGCGTGC | 58.70 | 59.23 | 237 | 140 |
100.00%
|
100.00%
|
| Primer 3 | Bacillus smithii | CGGAAGGGCTGTTCACTGT | ACGAATGCAGCCGTTGGA | 59.93 | 59.97 | 203 | 140 |
100.00%
|
100.00%
|
| Primer 4 | Bacillus smithii | TTGACCCAAGCACCAGACC | TTCGGGTGGTGCTTGATCC | 59.85 | 60.00 | 288 | 138 |
100.00%
|
100.00%
|
| Primer 5 | Bacillus smithii | GCACCGGTCAATGGATGGA | ACCCCAATGGCAACTGCA | 60.08 | 59.80 | 244 | 138 |
100.00%
|
100.00%
|
| Primer 6 | Bacillus smithii | TGTTCGGCTGGTTTGGAGG | ACCCCAATGGCAACTGCA | 60.23 | 59.80 | 277 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Bacillus smithii | TGCATCCGTTTCCCGTGT | TGCACGCATAGCACGACT | 59.57 | 59.74 | 286 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Bacillus smithii | ATGCATCCGTTTCCCGTGT | TGCACGCATAGCACGACT | 60.00 | 59.74 | 287 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Bacillus smithii | CATCCGTTTCCCGTGTGGA | TGCACGCATAGCACGACT | 60.00 | 59.74 | 284 | 138 |
100.00%
|
100.00%
|
| Primer 10 | Bacillus smithii | GGGTAAACGGTCAAGCGGA | TCGCTGCCGAATTGGACA | 60.00 | 59.65 | 201 | 137 |
100.00%
|
100.00%
|